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KMID : 0903519690110010055
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1969 Volume.11 No. 1 p.55 ~ p.61
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Studies on the Glucose Isomerizing Enzyme - Part Ⅲ The Effects of Metal Ions on the Reaction of K - 17 Strain´s Glucose Isomerizing Enzyme -
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Seu J. H.
Kim C. K.
Ki W. K.
Rhee In-Koo
Kwon T. J.
Woo D. L.
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Abstract
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This K-17 strain was not absolutely requiring xylose as an inducer for enzyme formations. The most activity of this enzyme was lost when treated at 75℃. for 30 hours but was not influenced at 70℃ for 70 hours of treatment. The activity of this enzyme was increased by the addition of magnesium ions or cobalt ions in the reaction system. In the studies, we found that the magnesium ions simply activate the enzyme reaction and the cobalt ions do not but protect the enzyme from heat inactivation. And it was also found the phosphate buffer solution was very suitable as glucose dissolving solvent on the enzyme reaction. The mixed carbon source medium containing glucose, fructose, sorbitol, xylose and sucrose was more favorable for enzyme production then a sole carbon source containing medium on the shaking culture method.
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